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KMID : 0360220070480030423
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Journal of the Korean Ophthalmological Society
2007 Volume.48 No. 3 p.423 ~ p.430
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Effects of Damaged Human Corneal Epithelial Cells on Differentiation of Human Mesenchymal Stem Cell
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Shin Mi-Sun
Hong Hyun-Sook
Son Young-Sook
Kim Jae-Chan
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Abstract
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Purpose: To identify the effects of microenvironmental changes caused by human corneal epithelial damages to characteristics or differentiation of human mesenchymal stem cells (hMSCs).
Methods: Artificial corneal damage was induced onto a cultured monolayer of human corneal epithelial cells. hMSCs were then co-cultured with damaged human corneal epithelial cells (dIHCE). Morphological changes in the co-cultured hMSCs were observed. To elucidate the differentiation of hMSCs into corneal keratocytes or epithelial cells, the expressions of α-smooth muscle actin, keratin-3/-12, and E-cadherin were confirmed by immunofluorescence.
Results: hMSCs co-cultured with dIHCE showed enhanced adherence in the neighborhood of dIHCE and morphological change into dendritic shapes at 6 days post-seeding. Although the expression of α-smooth muscle actin, known as hMSCs marker, significantly decreased at the dIHCE-contacted site of hMSCs; there were no expressional changes on keratin-3/-12 and E-cadherin, the markers of corneal epithelial cells. Interestingly, positive expression of corneal epithelial marker keratin-3/-12 was observed in dIHCE co-cultured hMSCs. hMSCs co-cultured with normal human corneal epithelial cells (nIHCE) were unable to attach, and showed no change in the expression of α-smooth muscle actin.
Conclusions: It is proposed that dIHCE causes a morphological change in hMSCs, and decreased expression of α-smooth muscle actin. These results suggest that dIHCE can affect a change in the characteristics and differentiation of hMSCs. J Korean Ophthalmol Soc 48(3):423-430, 2007
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KEYWORD
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Mesenchymal stem cell, Cornea epithelial cell, Coculture, α-smooth muscle actin
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